Loading...
 
3d browser

Erase Spots

This plug-in erases spots based on intensity-based criterions.
Several criterion can be added
List of criterions:

SNR: "Signal-to-Noise Ratio"

SNR is computed for each spot. If the SNR is inferior to the user-defined threshold, the spot is erased.
SNR = Spot intensity - Background intensity / Background Standard deviation
Spot intensity can be the mean value or a quantile within the spot
Background can be estimated either using voxels contained in the whole nuclear space, or the nuclear spaced without spots.

Absolute intensity

Spots with an intensity inferior to the threshold are erased