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TACGENE

 

 People Involved

Name Expertise 
Carine GIOVANNANGELIScientific CoordinatorStrategy and Design
Jean-Paul CONCORDETScientific and Technical CoordinatorStrategy and Design
Anne DE CIANTechnical CoordinatorARN/Protein and in vitro activities 
Alice BRIONEngineerCellular and Molecular Biology
Khadija LAMRIBETEngineerCellular and Molecular Biology

Contact: tacgene at mnhn.fr


Presentation

TACGENE was created in 2011 within the U1154-UMR7196, to facilitate access of academic laboratories to genome editing techniques. Supported under the National Project "Investissement d'Avenir" - National Infrastructures in Biology Health TEFOR (that stands for Transgenesis for Functional Studies on Model Organisms in English), we have developed a strong expertise in the design, production and use of TALEN (transcriptional Activated Effector like Nucleases) and CRISPR (clustered interspaced short palindromic Regularly repeat) / Cas.9 technologies. We use them in cell culture in our research projects and in collaboration in many model organisms, particularly rats, mice and zebrafish thanks to the Celphedia  Network.

The activities of research and development we carry out in GE2R team to improve these technologies, in parallel to TACGENE service activities enable us to offer advanced solutions and expertise on the various issues of genome editing (knock-out, knock-in ...) currently available.



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Nuclease technologies developped by TACGENE

Expertise and proposed Collaborative Services

  • Provision of protocols on request
  • Tips and genotyping assistance
  • In vitro activity of sgARN / purified Cas9 complexes
  • Production of Cas9 (mRNA and recombinant protein)
  • Transcription of sgRNAs and Quality Control
  • Transfections and activity assays in cells
  • Design and selection of sgRNAs
  • Design of a genome editing strategy according to the project

Publications involving the activities of TACGENE

  • Joutsen, J., Da Silva, A.-J., Luoto, J.C., Budzynski, M.A., Nylund, A.S., de Thonel, A., Concordet, J.-P., Mezger, V., Sabéran-Djoneidi, D., Henriksson, E. & Sistonen, L. Heat Shock Factor 2 Protects against Proteotoxicity by Maintaining Cell-Cell Adhesion . Cell Rep. 2020 Jan 14;30(2):583-597.e6. doi: 10.1016/j.celrep.2019.12.037. PubMed PMID: 31940498.
  • Khosravi, M.A., Abbasalipour, M.,Concordet, J.-P., Berg, J.V., Zeinali, S., Arashkia, A., Azadmanesh, K., Buch, T. & Karimipoor, M. Targeted deletion of BCL11A gene by CRISPR-Cas9 system for fetal hemoglobin reactivation: A promising approach for gene therapy of beta thalassemia disease. Eur J Pharmacol. 2019 Jul 5;854:398-405. doi: 10.1016/j.ejphar.2019.04.042. Epub 2019 Apr 27. PubMed PMID: 31039344.
  • Garcin, E.B., Gon, S., Sullivan, M.R., Brunette, G.J., Cian, A., Concordet J.-P.,Giovannangeli, C., Dirks, W.G., Eberth, S., Bernstein, K.A., Prakash, R., Jasin, M. & Modesti, M. Differential Requirements for the RAD51 Paralogs in Genome Repair and Maintenance in Human Cells . PLoS Genet. 2019 Oct 4;15(10):e1008355. doi:10.1371/journal.pgen.1008355. eCollection 2019 Oct. PubMed PMID: 31584931; PubMed Central PMCID: PMC6795472.
  • Dreano, E., Bacchetta, M., Simonin, J., Galmiche, L., Usal, C., Slimani, L., Sadoine, J., Tesson, L., Anegon, I., Concordet, J.-P., Hatton, A., Vignaud, L., Tondelier, D., Sermet-Gaudelus, I., Chanson, M. & Cottart, C.H.. Characterization of two rat models of cystic fibrosis-KO and F508del CFTR-Generated by Crispr-Cas9 . Animal Model Exp Med. 2019 Nov 25;2(4):297-311. doi: 10.1002/ame2.12091. eCollection 2019 Dec. PubMed PMID: 31942562; PubMed Central PMCID: PMC6930998.
  • Bastin-Héline, L., de Fouchier, A., Cao, S., Koutroumpa, F., Caballero-Vidal, G., Robakiewicz, S., Monsempes, C., François, M.-C., Ribeyre, T., Maria, A., Chertemps, T., de Cian, A., Walker, W.B. 3rd, Wang, G., Jacquin-Joly, E. & Montagné, N. A novel lineage of candidate pheromone receptors for sex communication in moths . Elife. 2019 Dec 10;8. pii: e49826. doi: 10.7554/eLife.49826. PubMed PMID: 31818368; PubMed Central PMCID: PMC6904214.
  • Concordet, J.-P. Haeussler, M. CRISPOR: intuitive guide selection for CRISPR/Cas9 genome editing experiments and screens. Nucleic Acids Res. 2018;46, W242–W245. https://doi.org/10.1093/nar/gky354
  • Ménoret, S., Ouisse, L.-H., Tesson, L., Delbos, F., Garnier, D., Remy, S., Usal, C., Concordet, J.-P. , Giovannangeli, C. , Chenouard, V., Brusselle, L., Merieau, E., Nerrière-Daguin, V., Duteille, F., Bellier-Waast, F., Fraichard, A., Nguyen, T.H., Anegon, I. Generation of Immunodeficient Rats With Rag1 and Il2rg Gene Deletions and Human Tissue Grafting Models. Transplantation. 2018;102, 1271–1278. https://doi.org/10.1097/TP.0000000000002251
  • Momose, T., De Cian, A. , Shiba, K., Inaba, K., Giovannangeli, C. , Concordet, J.-P. High doses of CRISPR/Cas9 ribonucleoprotein efficiently induce gene knockout with low mosaicism in the hydrozoan Clytia hemisphaerica through microhomology-mediated deletion . Sci Rep. 2018; 8, 11734. https://doi.org/10.1038/s41598-018-30188-0
  • Torbey, P., Thierion, E., Collombet, S., de Cian, A ., Desmarquet-Trin-Dinh, C., Dura, M., Concordet, J.-P. , Charnay, P., Gilardi-Hebenstreit, P. Cooperation, cis-interactions, versatility and evolutionary plasticity of multiple cis-acting elements underlie krox20 hindbrain regulation. PLoS Genet. 2018;14, e1007581. https://doi.org/10.1371/journal.pgen.1007581
  • Remy, S., Chenouard, V., Tesson, L., Usal, C., Ménoret, S., Brusselle, L., Heslan, J.-M., Nguyen, T.H., Bellien, J., Merot, J., De Cian, A. , Giovannangeli, C ., Concordet, J.-P. , Anegon, I. Generation of gene-edited rats by delivery of CRISPR/Cas9 protein and donor DNA into intact zygotes using electroporation . Sci Rep. 2017; 7, 16554. https://doi.org/10.1038/s41598-017-16328-y
  • Salama A, Mosser M, Leveque X, Perota A, Judor JP, Danna C, Pogu S, Moure A, Jegou D, Gaide N, Abadie J, Gauthier O, Concordet JP, Le Bas-Bernardet S, Riochet D, Le Berre L, Hervouet J, Minault D, Weiss P, Guicheux J, Brouard S, Bosch S, Lagutina I, Duchi R, Lazzari G, Cozzi E, Blancho G, Conchon S, Galli C, Soulillou JP, Bach JM. Neu5Gc and alpha1-3 GAL Xenoantigen Knockout Does Not Affect Glycemia Homeostasis and Insulin Secretion in Pigs. Diabetes .2017;66(4):987-993.
  • Koutroumpa F-A., Monsempes C., François M-C., De Cian A., Royer C., Concordet J-P. & Jacquin-Joly E. (2016) Heritable genome editing with CRISPR/Cas9 induces anosmia in a crop pest moth. Sci Rep. 6:29620.
  • Haeussler M., Schönig K., Eckert H., Eschstruth A., Mianné J., Renaud J-B., Schneider-Maunoury S., Shkumatava A., Teboul L., Kent J., Joly J-S. & Concordet J-P. (2016) Evaluation of off-target and on-target scoring algorithms and integration into the guide RNA selection tool CRISPOR. Genome Biol. 17(1):148.
  • Steed E., Faggianelli N., Roth S., Ramspacher C., Concordet J-P. & Vermot J. (2016) klf2a couples mechanotransduction and zebrafish valve morphogenesis through fibronectin synthesis. Nat Commun. 7:11646.
  • Haeussler M. & Concordet J-P. (2016) Genome Editing with CRISPR-Cas9: Can It Get Any Better? J. Genet. Genomics. 43,5:239-50.
  • Di Donato V., De Santis F., Auer T-O., Testa N., Sánchez-Iranzo H., Mercader N., Concordet J-P. & Del Bene F. (2016) 2C-Cas9: a versatile tool for clonal analysis of gene function. Genome Res. 26,5:681-92.
  • Böhm U-L., Prendergast A., Djenoune L., Nunes Figueiredo S., Gomez J., Stokes C., Kaiser S., Suster M., Kawakami K., Charpentier M., Concordet J-P., Rio J-P., Del Bene F. & Wyart C. (2016) CSF-contacting neurons regulate locomotion by relaying mechanical stimuli to spinal circuits. Nat. Commun. 7:10866.
  • Renaud J-B., Boix C., Charpentier M., De Cian A., Cochennec J., Duvernois-Berthet E., Perrouault L., Tesson L., Edouard J., Thinard R., Cherifi Y., Menoret S., Fontanière S., de Crozé N., Fraichard A., Sohm F., Anegon I., Concordet J-P. & Giovannangeli C. (2016) Improved Genome Editing Efficiency and Flexibility Using Modified Oligonucleotides with TALEN and CRISPR-Cas9 Nucleases. Cell Reports. 14,9:2263-72.
  • Menoret, S., De Cian, A., Tesson, L., Remy, S., Usal, C., Boulé, J-B., Boix, C., Fontanière, S., Crénéguy, A., Nguyen, T.,Brusselle, L., Thinard, R., Gauguier, D., Concordet, J-P., Cherifi, Y., Fraichard, A., Giovannangeli, C., & Anegon I. (2015) Homology-directed repair in rodent zygotes using Cas9 and TALEN engineered proteins. Sci Rep. 7(5):14410. 
  • Auer, T-O., Xiao, T., Bercier, V., Gebhardt, C., Duroure, K., Concordet, J-P., Wyart, C., Suster, M., Kawakami, K., Wittbrodt, J., Baier, H. & Del Bene, F. (2015) Deletion of a kinesin I motor unmasks a mechanism of homeostatic branching control by neurotrophin-3. Elife.15;4. doi: 10.7554/eLife.05061
  • Reinhardt, R., Centanin, L., Tavhelidse, T., Inoue, D., Wittbrodt, B., Concordet J-P., Martinez-Morales, J-R., & Wittbrodt, J. (2015) Sox2, Tlx, Gli3, and Her9 converge on Rx2 to define retinal stem cells in vivo. EMBO J. 3, 34(11):1572-88.
  • Auer, T-O., Duroure, K., Concordet, J-P. & Del Bene, F. (2014) CRISPR/Cas9-mediated conversion of eGFP- into Gal4-transgenic lines in zebrafish. Nat Protoc. 9,12: 2823-40.
  • Larcher, T., Lafoux, A., Tesson, L., Remy, S., Thepenier, V., François, V., Le Guiner, C., Goubin, H., Dutilleul, M., Guigand, L., Toumaniantz, G., De Cian, A., Boix, C., Renaud, J-B., Cherel, Y., Giovannangeli, C., Concordet, J-P., Anegon, I. & Huchet, C. (2014) Characterization of dystrophin deficient rats: a new model for Duchenne muscular dystrophy. PLoS One. 9,10: e110371.
  • Giovannangeli, C. & Concordet, J-P. (2014) Editing and investigating genomes with TALE and CRISPR/Cas systems: applications of artificial TALE and CRISPR-Cas systems. Methods. 69,2: 119-20.
  • Ghezraoui, H., Piganeau, M., Renouf, B., Renaud, J-B., Sallmyr, A., Ruis, B., Oh, S., Tomkinson, A-E., Hendrickson, E-A., Giovannangeli, C., Jasin, M. & Brunet, E. (2014). Chromosomal translocations in human cells are generated by canonical nonhomologous end-joining. Mol Cell. 55, 6: 829-42.
  • Rémy, S., Tesson, L., Ménoret, S., Usal, C., De Cian, A., Thepenier, V., Thinard, R., Baron, D., Charpentier, M., Renaud, J-B., Buelow, R., Cost, G-J., Giovannangeli, C., Fraichard, A., Concordet, J-P. & Anegon, I. Efficient gene targeting by homology-directed repair in rat zygotes using TALE nucleases. (2014) Genome Res. 24,8: 1371-83.
  • Ménoret, S., Tesson, L., Rémy, S., Usal, C., Thépenier, V., Thinard, R., Ouisse, L-H., De Cian, A., Giovannangeli, C., Concordet, J-P. & Anegon, I. (2014). Gene targeting in rats using transcription activator-like effector nucleases. Methods. 69,1: 102-7.
  • Auer, T-O., Duroure, K., De Cian, A., Concordet, J-P. & Del Bene, F. (2014). Highly efficient CRISPR/Cas9-mediated knock-in in zebrafish by homology-independent DNA repair. Genome Res, 24, 1:142-53.
  • Piganeau, M., Ghezraoui, H., De Cian, A., Guittat, L., Tomishima, M., Perrouault, L., René, O., Katibah, G-E., Zhang, L., Holmes, M-C., Doyon, Y., Concordet, J-P., Giovannangeli, C., Jasin, M. & Brunet, E.. (2013). Cancer translocations in human cells induced by zinc finger and TALE nucleases. Genome Res, 23,7 :1182-93.

Main Collaborations

  • Erika Brunet, MNHN, Paris (Chromosomal Translocations)
  • Ignacio Anegon,TRIP - UMR1064, Nantes (Rat Transgenesis)
  • Filippo Del Bene, Institut Curie, Paris (Genomic Targeting Zebrafish)
  • Frédéric Sohm, Amagen, Gif-sur-Yvette (Zebrafish Transgenesis)
  • Kristof Jagla,Fly-facility, Clermont-Ferrand (Drosophila Transgenesis)
  • Geneviève Tavernier, Anexplo, Toulouse (Mice Transgenesis)
  • Christophe Heligon, CRB Xénope, Rennes (Xenope Transgenesis)
  • Laure Bonnaud-Ponticelli, Guillaume Rivoire, UMR BOREA, MNHN
  • Hervé Tostivint, Barbara Demeinex, UMR 7221, MNHN (Genome editing in Zebrafish and Xenope)
  • Emmanuelle Jacquin-Joly, UMR 1272, INRA Versailles (Genome editing in Lepidoptera)
  • Tsuyoshi Momose, UMR7009, Villefranche s/ Mer (Genome editing in Clytia)
  • Rafael Dariolli, INCOR, Université Sao Paolo (Brésil)
  • Cesare Galli, Fondazione Avantea, Cremona (Italie)
  • Jochen Wittbrodt, Centre for Organismal studies, Heidelberg (Allemagne)

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